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Mitochondria (mthpp)

The main goal of this action is to obtain robust information about the integrative role of proteins acting at the mitochondrial level, considering both those encoded by the mitochondrial DNA (mt-DNA) and the nuclear encoded proteins (either imported into mitochondria or simply associated to them). Mitochondria are very versatile organelles and they are involved in a plethora of cellular functions, such as energy production, regulation of death pathways, calcium buffering. Therefore, their dysfunction has been related to many pathological conditions, ranging from neurodegenerative disorders to cancer and metabolic diseases. Hence their dynamic proteome is particularly important in defining their healthy state, the dynamics of the mitochondrial network and, as a consequence, cellular conditions.

    Current lines of work:

      • Standardization of mitochondrial proteomics on several cell lines by comparing three mitochondrial enrichment methods
      • Mitochondrial interactomics
      • Alterations of the mitochondrial proteome in neurodegenerative diseases
      • Mitochondrial degradomics

      Links:

      Papers:

      1. Urbani A, De Canio M, Palmieri F, Sechi S, Bini L, Castagnola M, Fasano M, Modesti A, Roncada P, Timperio AM, Bonizzi L, Brunori M, CutruzzolĂ  F, De Pinto V, Di Ilio C, Federici G, Folli F, Foti S, Gelfi C, Lauro D, Lucacchini A, Magni F, Messana I, Pandolfi PP, Papa S, Pucci P, Sacchetta P; Italian Mt-Hpp Study Group-Italian Proteomics Association (www.itpa.it). The mitochondrial Italian Human Proteome Project initiative (mt-HPP). Mol Biosyst. 2013 Aug;9(8):1984-92. doi: 10.1039/c3mb70065h. Epub 2013 May 28. PubMed PMID: 23712443.
      2. Alberio T, Bondi H, Colombo F, Alloggio I, Pieroni L, Urbani A, Fasano M. Mitochondrial proteomics investigation of a cellular model of impaired dopamine homeostasis, an early step in Parkinson's disease pathogenesis. Mol Biosyst. 2014 Jun;10(6):1332-44. doi: 10.1039/c3mb70611g. Epub 2014 Mar 28. PubMed PMID:24675778.
      3. Monti C, Bondi H, Urbani A, Fasano M, Alberio T. Systems biology analysis of the proteomic alterations induced by MPP(+), a Parkinson's disease-related mitochondrial toxin. Front Cell Neurosci. 2015 Feb 2;9:14. doi:10.3389/fncel.2015.00014. eCollection 2015. PubMed PMID: 25698928; PubMed Central PMCID: PMC4313704.
      4. Ciregia F, Giusti L, Ronci M, Bugliani M, Piga I, Pieroni L, Rossi C, Marchetti P, Urbani A, Lucacchini A. Glucagon-like peptide 1 protects INS-1E mitochondria against palmitate-mediated beta-cell dysfunction: a proteomic study. Mol Biosyst. 2015 Jun;11(6):1696-707. doi: 10.1039/c5mb00022j. PubMed PMID: 25912719.
      5. Fasano M, Alberio T, Babu M, Lundberg E, Urbani A. Towards a functional definition of the mitochondrial human proteome. EuPA Open Proteomics. 2016;10: 24-27. doi:10.1016/j.euprot.2016.01.004
      6. Di Pierro A, Bondi H, Monti C, Pieroni L, Cilio E, Urbani A, Alberio T, Fasano M, Ronci M. Experimental setup for the identification of mitochondrial protease substrates by shotgun and top-down proteomics. EuPA Open Proteomics. 2016;11: 1-3. doi:10.1016/j.euprot.2016.02.00.

      Links to other HPP groups:

      Mitochondrial C-HPP; neurodegeneration cluster; food and nutrition B/D-HPP.

      Future meetings and activities:

      • mtHPP meeting in June 2017, Lecce, Italy

      Leadership:

      Mauro Fasano, Chair

      For more information or participation opportunities please contact office(at)hupo.org.



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